Protéinase, bactérien, Type XXIV, 7,0 - 14,0 unités/mg solide, poudre lyophilisée

Code: p8038-1g D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.

Application

This is a proteolyti...


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£978.00 1G

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.

The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Packaging

1 g in glass bottle

50, 100, 250 mg in glass bottle

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 µmole (181 µg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

formlyophilized powder
mol wt27 kDa
purified bycrystallization
Quality Level300
shipped inwet ice
specific activity≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid, ≤0.05 Kunitz units/mg solid RNase
storage temp.−20°C
typeType XXIV
Code
Description
Unité de vente
Prix annoncé
Qté
P8038-100MG
Unité:100MG
Prix annoncé: £193.00
Source:Prix annoncé
P8038-250MG
Unité:250MG
Prix annoncé: £315.00
Source:Prix annoncé
P8038-50MG
Unité:50MG
Prix annoncé: £102.00
Source:Prix annoncé
Cas Number9014-01-1
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