Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

Used in the production of penicillin.

Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.

Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Unit Definition

One unit will hydrolyze 1.0 µmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.