Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Nuclease micrococcal from Staphylococcus aureus has been used in a study to monitor hybridization reactions with DNA. It has also been used in a study to investigate the optimal conditions for assay of staphylococcal nuclease.

Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes.

Biochem/physiol Actions

Hydrolyzes 5′-phosphodiester bonds of DNA.

Other Notes

Note: One µmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a δA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Packaging

50, 200, 500 units in glass bottle