Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Aldehyde Dehydrogenase, potassium-activated from yeast has been used:for enzyme immobilization and to oxidize formaldehyde to formateto study the functional relation betweenhydrazone to measure ethanol production.

Biochem/physiol Actions

Aldehyde dehydrogenase is a tetramer and has several different isoforms. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate.

Aldehyde dehydrogenase (ALDH)modulatesthenon-P450 aldehyde reduction enzyme system. It protects the cell from the effects of toxicaldehydes.Mutationsin this geneleads to Sjogren‐Larsson syndrome,Larsson syndrome, type IIhyperprolinemiaandcancer. ALDH-2lowerscardiac ischemia,whicharisesdue to myocardial infarction or post cardiac surgery.

General description

The family of aldehyde dehydrogenase (ALDH)contains19 genes in humans. It islocalized inthenucleus, cytosol, mitochondria and endoplasmic reticulum of thecell.

Aldehyde dehydrogenase, from yeast, is used to catalyze the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. Aldehyde dehydrogenase, from yeast has been used to study the production of ethanol and isobutanol. Aldehyde dehydrogenase, from Sigma, has been used along with alcohol dehydrogenase to measure ethanol production during characterization of glycolytic metabolism and ion transport in Candida albicans.

Packaging

25, 100, 250 units in poly bottle

Physical form

Contains potassium phosphate salts

Unit Definition

One unit will oxidize 1 micromole of acetaldehyde to acetic acid per minute pH 8.0 at 25 deg C.