Adenosine Deaminase de spleen bovin, Type IX, suspension de sulfate d'ammonium, 150-200 unités/mg de protéines

Code: a5168-250un D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

Adenosine deaminase is useful in various molecular biology assays, such as glycerol relea...


en savoir plus

Votre prix
$546.65 EACH

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculouspleural effusion. The activity of ADA is usually high in liver disease, tuberculosis,typhoid, infectivemononucleosisand several malignancies.

General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytesandmonocytes.

Molecular weight: 32.5-33 kDapI: 4.85

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0

Unit Definition

One unit will deaminate 1.0 µmole of adenosine to inosine per min at pH 7.5 at 25 °C.

foreign activitynucleoside phosphorylase ≤0.1%, 5′-AMP deaminase ≤0.01%, guanase ≤0.01%, alkaline phosphatase ≤0.01%
formammonium sulfate suspension
Gene Informationcow ... ADA(280712)
mol wt32.5-33 kDa
Quality Level200
specific activity150-200 units/mg protein
storage temp.2-8°C
typeType IX
UniProt accession no.P56658
Code
Description
Unité de vente
Prix annoncé
Qté
Cas Number21064-69-7
Ce produit répond aux critères suivants: