Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret

Application

Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem/physiol Actions

Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5′-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

General description

Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5′ protruding, recessive and blunt 5′-termini. The enzyme is irreversibly heat inactivated at 65°C for 15 minutes.

Physical form

solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl₂

Unit Definition

One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C.