Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteuriiandCanavalia ensiformis.

Biochem/physiol Actions

In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.

Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.

General description

Urease(Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.

Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620

Packaging

20000, 100000, 500000, 1000000 units in poly bottle

Unit Definition

One micromolar unit will liberate 1.0 µmole of NH₃ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)