Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.

Biochem/physiol Actions

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.

General description

Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe³⁺.

Packaging

25 units in glass bottle

Physical form

Supplied as lyophilized powder.

Physical properties

Structure : Protein with nonheme ironInhibitors : Ag⁺, Hg^++, PCMBOptimum pH : 9.0 Optimum temperature : 60−65°C pH Stability : pH 7.0−9.0 (25°C, 72hr) Thermal stability : below 50°C (pH 6.0, 1hr)

Unit Definition

One unit will oxidize 1.0 µmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.