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Analysis Note

Protein determined by biuret.

Application

Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.

Biochem/physiol Actions

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ~7.5 Optimal Temperature: 25°C εA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase from rabbit muscle can be activated by histidine and inhibited by low levels of zinc (Zn²⁺).

General description

Pyruvate kinase from rabbit muscle catalyzes an ATP-dependent phosphorylation of glycolate to yield 2-phosphoglycolate.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 6

Unit Definition

One unit will convert 1.0 µmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.