Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret.

Application

Lipase from Candida rugosa has been used in a study to assess the influence of phase segregation on enzyme hydrolytic activity.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Lipase from Candida rugosa has been used:in the hydrolysis of loaded polycaprolactone–20% tricalcium phosphate (PCL–TCP) compositesin adsorption of proteins in emulsan/alginate beads and in enzyme assaysto catalyse methanolysis and hydrolysis reactions

Lipase from C. rugosa is used to catalyze the synthesis of flavor esters such as pentyl propanoate and butyl butanoate used in food enhancement.

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Lipases are used in detergent, food, leather, pharmaceutical and diary industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

General description

Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Packaging

1000000, 5000000 units in poly bottle

Unit Definition

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.2 at 37 °C using olive oil (30 minute incubation).