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Analysis Note

Protein determined by biuret

Application

L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay.

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

General description

Lactic dehydrogenase (LDH) catalyzes the conversion of lactate to pyruvate in anaerobic glycolysis. It exists as tetramer and comprises of two subunits (H and M). The LDH of eukaryotes undergo active-site loop gating for their catalytic functionality.

Physical form

Crystalline suspension in 2.1 M (NH₄)₂SO₄ solution, pH 6.0

Unit Definition

One unit will reduce 1.0 µmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.