Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Protein determined by biuret

Application

L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca²⁺-ATPase (SERCA).

Biochem/physiol Actions

Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Variation in the levels of L-Lactic Dehydrogenase (LDH) isoform is a diagnostic marker for tissue damage. The levels of LDH is useful in detecting dairy cattle disease, Mastitis. Dye based purified LDH from bovine heart has analytical applications.

General description

L-Lactic Dehydrogenase (LDH) is present in the cell cytoplasm and is part of the glycolytic pathway. It exists in different isoforms in heart and muscles. LDH is a tetramer corresponding to a molecular weight of 140 kDa. LDH has a nucleotide-cofactor binding site and has the structure of Rossmann fold.

Packaging

12500 units in glass bottle

Physical form

Solution in 50% glycerol containing 0.025 M potassium phosphate buffer, pH 7.5

Unit Definition

One unit will reduce 1.0 µmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.