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Analysis Note

Protein determined by biuret.

Application

α-Glycerophosphate Dehydrogenase from rabbit muscle has been used:in the reaction mixture to measure the glycerol kinase activityto demonstrate compartmentalized enzymatic reactions where NADH is involvedin the reaction mixture assay for L-fuculose-1-phosphate aldolase

Biochem/physiol Actions

α-Glycerophosphate dehydrogenase catalyzes the oxidation of L-α-glycerophosphate (Glp) to form dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H2O2). This enzyme is associated with respiratory electron transport chain, glycolysis, and phospholipid metabolism.

General description

α-Glycerophosphate dehydrogenase is a membrane protein and a flavin-linked primary dehydrogenase.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ and 0.1 g/L EDTA solution, pH 6.0

Preparation Note

Prepared by modification of the method of Fondy.

Unit Definition

One unit will convert 1.0 µmole of dihydroxyacetone phosphate to α-glycerophosphate per min at pH 7.4 at 25 °C.