Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Urease fromCanavalia ensiformis(Jack bean) has been used in purification and crystallization studies.

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

General description

Subunit molecular weight: ~90,770 Composed of six subunits with total molecular weight: ~544,620

Urease(Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Ureaseconsists of nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.

Other Notes

Application in (selective) hydrolysis/condensations; structure.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol urea (Cat. No. 51459) per minute at pH 8.0 and 25 °C.