5-Bromo - 4 - chloro - 3-phosphated'indolyle - sel P - toluidine, bioréactif, pour biologie moléculaire, poudre, >=99 %

Code: b6777-100mg D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are availab...


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Votre prix
£108.00 100MG

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

Histochemical substrate for alkaline phosphatase.

assay≥99%
foreign activityProtease, none detected
formpowder
gradefor molecular biology
InChI keyQEIFSLUFHRCVQL-UHFFFAOYSA-N
InChI1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
product lineBioReagent
Quality Level200
SMILES stringCc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
solubilityDMF: 20 mg/mL, H2O: insoluble
storage temp.−20°C
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Description
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Cas Number6578-06-9
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