Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.
Ce produit répond aux critères suivants: