Application

The E. coli strain BL21 (DE3) has been used to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.

Components

BL21(DE3) chemically competent cells pUC 19 transformation control DNA recovery medium for expression

Features and Benefits

a transformation efficiency of >1 × 10⁷ cfu/µg. economical prices offering superior value for everyday protein expression work.

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.GenotypeF – ompT hsdSB (rB- mB-) gal dcm (DE3)