Application

Trypan blue has been used:in cell viability assayto determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.to stain plant tissue in order to to visualize cell death

Biochem/physiol Actions

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

General description

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Packaging

5, 25, 100 g in glass bottle