Huile minérale, huile légère (pure), bioréactif, convient à la culture de cellules embryons de souris

Code: m8410-500ml D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids ...


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Votre prix
£34.50 500ML
£41.40 inc. VAT

Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

Application

Mineral oil has been used as an overlay in microdrop embryo cultures.

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Packaging

1 L in glass bottle

100, 500 mL in glass bottle

5 mL in poly bottle

density0.84 g/mL at 25 °C (lit.)
formlight oil
formatneat
InChI keyAEOVEGJBKQQFOP-DDVLFWKVSA-L
Quality Level200
refractive indexn20/D 1.467 (lit.)
sterilitynon-sterile
storage conditionprotect from light
Code
Description
Unité de vente
Prix annoncé
Qté
Cas Number8042-47-5
Pack500ML
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