G 418 SOLUTION DE SEL DE DISULFATE

Code: g8168-50ml D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal ...


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Votre prix
$498.80 50ML

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.

Biochem/physiol Actions

Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.

Caution

The product is stable for two years when stored at 2-8°C.

General description

Chemical structure: aminoglycoside

antibiotic activity spectrumparasites
biological sourceMicromonospora echinospora
colorcolorless
concentration50 mg/mL in H2O
formsolution
impuritiesendotoxin, tested
InChI keyUHEPSJJJMTWUCP-NKCAIAFTSA-N
InChI1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1
mode of actionprotein synthesis | interferes
mol wt692.7
packagingpkg of 10 and 100 mL
potency50-60 mg per mL
product lineBioReagent
Quality Level200
SMILES stringOS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
solubilityH2O: 50 mg/mL, clear
sterility0.1 µm filtered
storage temp.2-8°C
technique(s)cell culture | mammalian: suitable
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Pack50ML
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