Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 µg/mL or less while animal cells may require 300-500 µg/mL.
Biochem/physiol Actions
Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Caution
The product is stable for two years when stored at 2-8°C.
General description
Chemical structure: aminoglycoside
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