x100-1l

Application

Triton^™-X has been used:In the permeabilization of cells for immunofluorescence stainingAs a component of lysis buffer in western blot analysisAs a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Packaging

5 mL in glass bottle

100, 500 mL in poly bottle

1 gal in poly bottle

1 L in poly bottle

5 gal in poly drum

aggregation number	100-155
CMC	0.2-0.9 mM (20-25°C)
description	non-ionic
form	liquid
grade	laboratory grade
HLB	13.5
InChI key	HNLXNOZHXNSSPN-UHFFFAOYSA-N
InChI	1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
mol wt	micellar avg mol wt 80,000, average mol wt 625
Quality Level	200
SMILES string	CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
solubility	water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
transition temp	cloud point 65 °C, pour point ~7 °C