Application

Triton^™ X-100 has been used:In immunohistochemistry for staining the Flat-mount retinasAlong with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assayTo permeabilise cells during Immunofluorescent microscopic studiesAs a positive control in LDH assay to determine the cell membrane integrityFor estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedureFor the preparation of outer membrane protein exctractAs a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cellsIn the treatment of tissue sections for Immunofluorescence labeling

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

General description

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Packaging

50, 100, 250 mL in poly bottle