Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Trizma^® hydrochloride solution may be used in the following studies:As buffer for the 2-D electrophoresis of rat fibroblast cell.As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

General description

Trizma^® hydrochloride solution is a useful biological buffer.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 µm filtered.