Not available outside of the UK & Ireland.

General description

Triton™ X-100 has hemolytic property and is used for DNA extraction. Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilise proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilised for lysing cells to extract protein and cellular organelles. It can also permeabilise the living cell membrane for transfection.

Application

Triton™-X has been used:

• In the permeabilisation of cells for immunofluorescence staining
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Biochem/physiol actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and benefits

• Ideal for cell biology and biochemical research applications
• Non-ionic non-denaturing detergent
• Superior wetting agent and emulsifier
• Highly versatile surfactant