f23220

Not available outside of the UK & Ireland.

General description

Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilise proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilised for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton™ X-100 has been used:

For permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
As a component of lysis buffer used for homogenisation of cells
As a component of blocking buffer in immunofluorescence staining
For the preparation of cells in double fluorescence staining

Biochem/physiol actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Features and benefits

Suitable for molecular biology and biochemical research
Free from DNase, RNase, NICKase and protease
Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
High purity product for research applications
Tested for the presence of anionic and cationic traces

absorption	cut-off at 310 nm
aggregation number	100-155
cation traces	Cd: ≤1 mg/kg, K: ≤100 mg/kg, Fe: ≤1 mg/kg, Mg: ≤1 mg/kg, Bi: ≤1 mg/kg, Mo: ≤1 mg/kg, Al: ≤1 mg/kg, Na: ≤100 mg/kg, Ni: ≤1 mg/kg, Cr: ≤1 mg/kg, Ca: ≤5 mg/kg, Zn: ≤1 mg/kg, Pb: ≤1 mg/kg, Co: ≤1 mg/kg, Ba: ≤1 mg/kg, Cu: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Li: ≤1 mg/kg
CMC	0.2-0.9 mM (20-25°C), 0.23 mM
concentration	~10% in H₂O
density	1.01 g/mL at 20 °C
description	non-ionic
form	liquid
grade	for molecular biology
HLB	13.5
impurities	phosphatases, none detected, RNases, none detected, proteases, none detected, insoluble matter, passes filter test, DNases, none detected
InChI key	HNLXNOZHXNSSPN-UHFFFAOYSA-N
InChI	1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
mol wt	micellar avg mol wt 80,000, average mol wt 625
pH	6.5-8.5 (25 °C)
product line	BioUltra
Quality Level	200
refractive index	n20/D 1.349±0.002
SMILES string	CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
transition temp	cloud point 65 °C, pour point ~7 °C