Non disponible en dehors du Royaume-Uni et de l'Irlande
Application
Triton-X has been used:for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometryAs a component of lysis buffer used for homogenization of cellsAs a component of blocking buffer in immunofluorescence stainingFor the preparation of cells in double fluorescence staining
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Ce produit répond aux critères suivants: