Bromobimane, bioréactif, adapté à la fluorescence, >=95% (HPCE)

Code: 69898-25mg D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-...


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Votre prix
$198.65 25MG

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Bromobimane is used for the determination of thiols by the HPLC method. It is suitable as a pre-column derivatization agent for fluorometric determination of 2,3-dimercaptopropane-1-sulfonic acid and other dithiols. Bromobimane has been used as a fluorescent label in studying oligomycin-sensitive ATPase from beef heart mitochondria.

Features and Benefits

The benefits of using Bromobimane for the analysis of low molecular weight thiols are: Highly selective. Rapid reactivity. Ease of separation of derivatives by reverse HPLC. Ability to penetrate cells.

General description

Bromobimane, also called probe for thiols, is a fluorescent reagent activated upon a photolysis reaction. Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH. And, upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

Other Notes

Fluorescent thiol-specific reagent

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

assay≥95% (HPCE)
fluorescenceλex 398 nm, λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
formsolid
InChI keyAHEWZZJEDQVLOP-UHFFFAOYSA-N
InChI1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
mp152-154 °C (lit.)
product lineBioReagent
Quality Level100
SMILES stringCC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
solubilitymethanol: soluble, DMF: soluble, acetonitrile: soluble, DMSO: soluble
storage temp.2-8°C
suitabilitysuitable for fluorescence
Code
Description
Unité de vente
Prix annoncé
Qté
69898-100MG
Unité:100MG
Prix annoncé: $652.50
Source:Prix annoncé
Cas Number71418-44-5
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