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Application

Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto Rho6G are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.

General description

Atto Rho6G is a novel fluorescent label that belongs to the class of Rhodamine dyes. It shows a strong absorption, extraordinary high fluorescence quantum yield, high thermal and photostability, and a very little triplet formation. Atto Rho6G is moderately hydrophilic.Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.