Not available outside of the UK & Ireland.

Application

Anti-Mouse IgG-Abberior^® STAR 580 antibody produced in goat has been used for STED (stimulated emission depletion) microscopy in CA3 (Cornus Ammonis) sections. Abberior^® STAR 580 conjugated with secondary antibody has been used for dual-color STED imaging of cultured hippocampal neurons.

General description

Abberior STAR 580 is the latest development for STED microscopy with a fluorescent dye in the orange regime. The dye can be excited from 550 to 580 nm. Abberior STAR 580 can substitute dyes like Atto^™ 590/594 or AlexaFluor 584/594. The dye can most effectively be depleted in STED microscopy at 700 to 780 nm via e.g. a Ti:Sa laser or IR diodes.Abberior STAR 580 is the dye of choice for orange fluorescence. Moreover, the dye is particularly designed and tested for 2-color STED microscopy in combination with Abberior STAR 635 using 2 separate STED wavelength. This offers the great advantage of using 2 regular (not featuring a long Stokes-shift) dyes, which are in general more photostable. Please see our 2-color dye section Key features Exceptionally bright orange fluorescent dye Ideal for STED microscopy at 700-775 nm 2-color labeling partner with STAR 635P for 2-color STED microscopyAbsorption Maximum, λmax:: 587 nm (PBS, pH 7.4; water) 584 nm (MeOH, aq. ACN) Extinction Coefficient, ε(λmax): 85,000 M^-1cm^-1 (PBS, pH 7.4)Correction Factor, CF260 = ε260/εmax: 0.17 (PBS, pH 7.4; water)Correction Factor, CF280 = ε280/εmax: 0.15 (MeOH, aq. ACN)Fluorescence Maximum, λfl: 607 nm (PBS, pH 7.4) 604 nm (MeOH, aq. ACN)Recommended STED Wavelength, λSTED: 700 - 780 nm Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)Fluorescence Lifetime, τ: 3.5 ns (PBS, pH 7.4)

Legal Information

abberior is a registered trademark of Abberior GmbH

Atto is a trademark of Atto-Tec GmbH

Other Notes

Find more infomation here Abberior^®

Suitability

Designed and tested for fluorescent super-resolution microscopy