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Application

Abberior^® STAR 440SXP has been conjugated with secondary anti-mouse antibody for dual colour STED (stimulated emission depletion) microscopy. Again, it has been labelled with secondary antibody for STED microscopy in primary hippocampal cells prepared from E18 Sprague Dawley embryos.

General description

Absorption Maximum, λmax: 433 nm (MeOH) 432 nm (PBS, pH 7.4) Extinction Coefficient, ε(λmax): 36,000 M^-1cm^-1 (MeOH) 33,000 M^-1cm^-1 (PBS, pH 7.4)Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4) Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4) Fluorescence Maximum, λfl: 502 nm (MeOH), 511 nm (PBS, pH 7.4) Recommended STED Wavelength, λSTED: 590 − 620 nm Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4) Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

Legal Information

abberior is a registered trademark of Abberior GmbH

Other Notes

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Suitability

Designed and tested for fluorescent super-resolution microscopy