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General description

Abberior STAR635P is the first member of an entirely new class of dyes introduced exclusively by Abberior - first commercially available phosphorylated fluorescent dye. The dye excels in its photophysical parameters, e.g. very bright, very stable and it enables basically background free imaging.Abberior STAR 635P can substitute Atto^™ 647N, AlexaFluor 647, or Cy5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength of 750 - 780 nm is recommended. Please see reference1 for detailled characteristics.Best results are obtained with freshly prepared samples.Abberior STAR 635P particularly well suits the Leica TCS STED system and delivers high-resolution STED images with unmatched quality, e.g. extremely low/no background. The dye serves as an ideal partner for several 2-color STED.Key Features First phosphorylated dye Unmatched, background free STED imaging contrast Verified in the Leica STED systemAbsorption Maximum, λmax: 638 nm (PBS, pH 7.4)Extinction Coefficient, ε(λmax): 120′000 M^-1cm^-1 (PBS, pH 7.4) Correction Factor, CF260 = ε260/εmax: 0.21 (PBS, pH 7.4)Correction Factor, CF280 = ε280/εmax: 0.40 (PBS, pH 7.4)Fluorescence Maximum, λfl: 651 nm (PBS, pH 7.4)Recommended STED Wavelength, λSTED: 750 - 780 nm Fluorescence Quantum Yield, η: 0.90 (PBS, pH 7.4)Fluorescence Lifetime, τ: 3.3 ns (PBS, pH 7.4)

Legal Information

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Suitability

Designed and tested for fluorescent super-resolution microscopy