Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Anti-Myosin 7 (MYH7), clone BA-D5, Cat. No. MABT838, is a mouse monoclonal antibody that detects Myosin-7 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Research CategoryCell Structure

Immunohistochemistry Analysis: A representative lot detected Myosin 7 (MYH7) in Immunohistochemistry applications (Goh, Q., et. al. (2017). Elife. 6. pii: e20007).

Western Blotting Analysis: A representative lot detected Myosin 7 (MYH7) in Western Blotting applications (De Jong, A., et. al. (2017). PLoS One. 12(3):e0174043; Gan, Z., et. al. (2013). J Clin Invest. 123(6):2564-75).

Immunofluorescence Analysis: A representative lot detected Myosin 7 (MYH7) in Immunofluorescence applications (Gan, Z., et. al. (2013). J Clin Invest. 123(6):2564-75; Pannerec, A., et. al. (2016). Aging (Albany NY). 8(4):712-29).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myosin-7 (UniProt: Q9BE39; also known as Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, Myosin heavy chain, cardiac muscle beta isoform, MyHC-beta) is encoded by the MYH7 gene (Gene ID: 282714) in bovine species. Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Myosins form regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle. Myosin-7 is a component of thick filaments of the myofibrils. It contains actin- and ATP-binding sites in its conserved catalytic head domain. Its actin binding region is localized to amino acids 655-677 and 757-771 and the ATP-binding region is localized to amino acids 178-185. Myosin-7 has an N-terminal SH3-like domain (aa 32-81), a myosin motor domain (aa 85-778), and an IQ domain (aa 781-810). Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces one light meromyosin (LMM) and one heavy meromyosin (HMM). HMM can be further cleaved into two globular sub fragments (S1) and 1 rod-shaped sub fragment (S2).

Immunogen

Purified myosin from bovine atrium.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse soleus muscle tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected Myosin 7 (MYH7) in mouse soleus muscle tissue lysate.

Specificity

Clone BA-D5 detects Myosin-7 in multiple mammalian species. It detects both alpha- and beta-slow myosin heavy chain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~220 kDa observed; 223.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).