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Analysis Note

ControlHuman platelets cell lysate

Application

Research Sub CategoryIntegrins

Research CategoryCell Structure

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Ginsberg, M., et al. (1990). Blood. 76:2017-2023.)

Anti-LIBS2 epitope (Integrin beta3 subunit) Antibody, clone ab62 is an antibody against LIBS2 epitope (Integrin beta3 subunit) for use in WB, FC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue.
Clone Ab62 is a monoclonal antibody that specifically recognizes the LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit (Wiberzbicka-Patynowski, I., et al. (1999). JBC. 274(53):37809-37814).

Immunogen

Human GPIIa-IIIb purified from platelets

Epitope: LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit of integrins.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human platelets cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected LIBS2 domain of integrin beta3 subunit in 10 µg of human platelets cell lysate.

Specificity

This antibody recognizes the LIBS2 epitope in the C-terminal region of the extracellular domain of the beta3 subunit of integrins.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~110 kDa observed in human platelets.
Other MW bands may vary or may be observed depending on lysate used and if the LIBS2 epitope is present in other proteins.