Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Ago2 in HEK239 cell lysate (Rudel, S., et al. (2008) RNA. 14(6):1244-1253.).

Immunocytochemistry Analysis: A represeentative lot from an independent laboratory detected Ago2 in HEK293, HeLa, and RPE-1 cells (Weinmann, L., et al. (2009). Cell. 136(3):496-507.).

Use Anti-Ago2 Antibody, clone 11A9 (Rat Monoclonal Antibody) validated in WB, IP, ICC to detect Ago2 also known as Protein argonaute-2, Argonaute2, hAgo2, eIF-2C 2.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ago2 (argonaute-2), also known as eukaryotic translation initiation factor 2C (EIF2C2), is an essential component for siRNA-directed RNA interference (RNAi) response. Ago2 is an endonuclease required for the unwinding of siRNA duplex and assembly of siRNA into RNA-induced silencing complexes (RISC). Ago2 interacts with DICER1 through its Piwi domain. This Piwi domain is thought to provide RNA cleavage activity via a mechanism similar to RNase H. Ago2 activity is necessary for embryonic development as well as RNA-mediated gene silencing (RNAi).

Immunogen

Linear peptide corresponding to human Ago2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected Ago2 in 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85 kDa observed. Two isoforms at ~97 kDa and ~94 kDa are known to exist.