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Application

Research Sub CategoryApoptosis - AdditionalCell Cycle, DNA Replication & Repair

Research CategoryApoptosis & Cancer

Anti-DNA Antibody, single stranded, clone 16-19 is an antibody against DNA for use in ELISA.

ELISA
This antibody is evaluated using ELISA on single stranded DNA from Calf Thymus.
Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: single stranded DNA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Format: Purified

0.1 M Tris-Glycine (pH 7.4), 150 nM NaCl with 0.05% sodium azide.

Specificity

Single stranded DNA (ssDNA) (poly-deoxythymidine). Shows 90% reactivity to ssDNA and 12% reactivity to dsDNA.

ANTIGEN BINDING PROFILE:

Relative Binding To:

Natural DNA Synthetic homopolymers

dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)

0.03 0.84 0.02 0.11 0.03 1.00 0.03 0.53

a) Native calf thymus DNA
b) Heat-denatured calf thymus DNA

Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 µg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).

BINDING ASSAY

Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) for one hour at room temperature, followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.

ISOELECTRIC POINT = 8.3

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.