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Application

IgG2a FITC Isotype Control from murine myeloma has been used in dual-colour staining and in cell staining for flow cytometry.

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgG2a (immunoglobulin G 2a) is one of the subclasses of rat immunoglobulins, which also include IgG1, IgG2b and IgG2c.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Specificity

Mouse IgG2a, κ, antibodies react with anti-mouse whole serum, anti-mouse IgG2a and anti-mouse κ chain immunoglobulins. The antibody preparation, IgG2a, does not react with antisera to mouse IgA, IgM, IgG1, IgG2b, IgG3 and λ light chain immunoglobulins in Ouchterlony double diffusion assays. IgG2a-FITC conjugate can be used as controls in immunoassays.