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Analysis Note

ControlA20 cell lysate

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TSLP in human prostate cancer and heart tissues.

Immunofluorescence Analysis: A 20 µg/mL from a representative lot detected TSLP in human brain cells.

Detect Thymic stromal lymphopoietin using this rabbit polyclonal antibody, Anti-TSLP Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. TSLP is expressed in a number of tissues including heart, liver, and prostate.

Immunogen

KLH-conjugated linear peptide corresponding to the intermediate domain of human TSLP.

Epitope: Intermediate domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TSLP in 15 µg of A20 cell lysate.

Specificity

This antibody recognizes the intermediate domain of TSLP.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~23 kDa observed. The calculated molecular weight of this protein is 18 kDa, but can be observed at ~23 kDa due to high glycosylation.