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Analysis Note

ControlMouse E13.5 embryo tissue lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in mouse E14.5 telencephalon tissue. (Image courtesy of Dr. Kenneth Campbell, Division of Developmental Biology, Cincinnati Children′s Hospital Medical Center.)

Anti-Gsh2 Antibody is an antibody against Gsh2 for use in Western Blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Gsh2 is a homedomain transcription factor that is critically involved in neurogenesis during embryonic development. It is involved in the development of the ventral forebrain structures from lateral ganglionic eminence (LGE) neuronal progenitor cells. Gsh2 may also delineate boundaries between cell types and control cell proliferation. Gsh2 functions downstream of the sonic hedgehog (Shh) pathway.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of mouse Gsh2.

Epitope: C-terminus

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in mouse E13.5 embryo tissue lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected Gsh2 on 10 µg of mouse E13.5 embryo tissue lysate.

Specificity

This antibody recognizes the C-terminus of Gsh2.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~35 kDa observed