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Analysis Note

ControlEmbryonic Stem Cell lysates

Application

Research Sub CategoryTranscription Factors

Anti-Zscan4 Antibody is a highly specific rabbit polyclonal antibody, that targets Zinc Finger Protein & has been tested in western blotting, Immunofluorescence & IP.

Research CategoryEpigenetics & Nuclear Function

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Zscan4 in mouse iPS and R1 Embyonic Stem cells. Data courtesy of the Melanie Welham lab at The University of Bath, UK
Immunoprecipitation Analysis: A 1:4,000 dilution from a representative lot detected Zscan4 in cytoplasmic and nuclear extracts in IP experiments conducted by the Melanie Welham lab at The University of Bath, UK. (Data not shown)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger and SCAN domain containing protein 4, also called Zscan4, is an embryonic stem (ES) cell-specific transcription factor required to regulate pluripotency. Zscan4 binds and regulates telomere elongation in order to maintain genomic stability and acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) in undifferentiated ES cells.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Zscan4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse Embryonic Stem cell lysates.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Zscan4 in 10 µg of Embryonic Stem cell lysates.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed. The calculated molecular weight of this protein is 57 kDa An uncharacterized band may be observed at ~50 kDa in some cell lysates.ZSCAN4 bands may be observed at both ~72 kDa and ~40 kDa when preparing cell lysates with SDS buffer. Additionally, a ZSCAN4 doublet may be observed at ~72 kDa when using cytosolic or nuclear extracts.