Antiactin Monoclonal alpha-lisse Muscle- Cy3 anticorps produit dans la souris, clone 1 A4, immunoglobulin purifié, solution aqueuse tamponnée

Code: c6198-.2ml D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. IHC analysis of x-gal st...


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Non disponible en dehors du Royaume-Uni et de l'Irlande

Application

Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin. IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.

Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Flow cytometry/Cell sorting (2 papers)Immunocytochemistry (3 papers)Immunofluorescence (13 papers)Immunohistochemistry (27 papers)Western Blotting (2 papers)

Biochem/physiol Actions

Smooth muscle actin is transiently coexpressed with sarcomeric α-actin during myogenesis in chicken and rat embryos. It has been found in the ventricular conducting tract of adult mammalian heart. It is expressed in leiomyomas, leiomyosarcomas and leiomyoblastomas, as well as in a proportion of rhabdomyosarcomas.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Monoclonal Anti-Actin, α-Smooth Muscle (mouse IgG2a isotype) is derived from the 1A4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice.

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Legal Information

Cy3 is a trademark of Cytiva

Cy is distributed under license from Amersham Biosciences Limited.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
application(s)research pathology
biological sourcemouse
clone1A4, monoclonal
conjugateCY3 conjugate
formbuffered aqueous solution
Gene Informationhuman ... ACTA2(59)mouse ... Acta2(11475)rat ... Acta2(81633)
isotypeIgG2a
mol wtantigen ~42 kDa
Quality Level200
shipped inwet ice
species reactivityhuman, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake
storage conditionprotect from light
storage temp.2-8°C
technique(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil or appendix
UniProt accession no.P62736
Code
Description
Unité de vente
Prix annoncé
Qté
C6198-100UL
Unité:100UL
Prix annoncé: $536.50
Source:Prix annoncé
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