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Application

Research CategoryNeuroscience

Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Gene Product 9.5 in human cerebellum.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Gene Product 9.5 in E18 rat cortical cells..

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Research Sub CategoryDevelopmental Neuroscience

Detect PGP9.5 using this rabbit polyclonal antibody, Anti-Protein Gene Product 9.5 Antibody validated for use in western blotting, IHC (Paraffin), Immunofluorescence & ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down-regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.

Immunogen

KLH-conjugated linear peptide corresponding to human Protein Gene Product 9.5.

Legal Information

DyLight is a registered trademark of Pierce Biotechnology, Inc.

Linkage

Replaces: AB1761 and AB9724

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH7.4 with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in rat brain, human brain, and mouse brain tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Protein Gene Product 9.5 (UCH-L1) in rat brain, human brain, and mouse brain tissue lysate

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~24 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.