Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.

General description

Recombinant, human, interleukin-6 expressed in E. coli. Interleukin-6 is a protein produced by a number of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity. Stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B-cells, T-cells, and hepatocytes. Stimulates growth and inhibits constitutive protein synthesis-independent apoptosis of murine B-cell hybridoma 7TD1. Human sequence IL-6 is active on murine cells. 1 µg is equivalent to 7.0 x 10³ WHO units.

Recombinant, human, interleukin-6 expressed in E. coli. A prototypic pleiotrophic, pro-inflammatory cytokine that stimulates the production of acute phase proteins by hepatocytes. Also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is produced by macrophages, endothelial cells, and activated T helper cells. Also considered as a potential marker of adipocyte differentiation. Human IL-6 is active on murine cells.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9.Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890.Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.Liu, J., et al. 1994. Cell Immunol.155, 229.Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.

Packaging

10 µg in Plastic ampoule

Physical form

Carrier free.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.

Warning

Toxicity: Standard Handling (A)