Non disponible en dehors du Royaume-Uni et de l'Irlande

Analysis Note

Positive ControlHeLa cells

Application

Immunoblotting (10-500 ng/ml, chemiluminescence)r>Immunocytochemistry (5 µg/ml)r>Immunoprecipitation (see comments)

General description

Recognizes the ~17 kDa acetylated histone H3 protein.

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.

Immunogen

Human

a diacetylated synthetic peptide [ARTKQTAR_AcKSTGG_AcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser¹⁰ on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349.Braunstein, M., et al. 1993. Genes Devel.7, 592.Perry, C.A., et al. 1993. Biochemistry32, 13605.Lin, R., et al. 1989. J. Cell. Biol.108, 1577.

Packaging

50 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)