Glucose Oxidase; Aspergillus niger ; Recombinant

Code: 345386-10ku D2-231

Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-Ω-l...


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Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-Ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-Ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-Ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-Ω-lactone to gluconic acid. Useful for enzymatic assay of glucose concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

Packaging

10 ku in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Warning

Toxicity: Standard Handling (A)

coloryellow-brown
foreign activitycatalase ≤20 U/mg (dry weight), sucrase ≤0.1%, Amylase ≤0.1%
formlyophilized solid
manufacturer/tradenameCalbiochem®
Quality Level100
shipped inambient
solubility0.1 M phosphate buffer, pH 7.0: 5 mg/mL
specific activity≥250 units/mg solid
storage conditionOK to freeze
storage temp.2-8°C
Cas Number9001-37-0
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