Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.

General description

Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His Tag™ sequence. Both the GST moiety and the His Tag™ sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺ and Mg²⁺ for optimal activity.

Recombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His Tag™ sequence. Both the GST moiety and the His Tag™ sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn²⁺ and Mg²⁺ for optimal activity.

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zimmerman, K.K., et al. 1998. Protein Expr. Purif.14, 395.Reiss, Y., et al. 1990. Cell62, 81.

Packaging

100 µg in Plastic ampoule

Physical form

In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)