Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

≥1,200 Factor BH50 units/mg; ≥50% of Factor B activity in NHS (mg/mg basis)

General description

Factor B, Human, is a single-chain glycoprotein present in normal human serum that is cleaved during the activation of complement via the alternative pathway into two fragments of 30 and 63 kDa.

Native factor B from human serum. Single-chain glycoprotein present in normal human serum at about 200 µg/ml. During activation of complement via the alternative pathway, Factor B is cleaved, and thus activated by Factor D into two fragments of M.W. of ~30 kDa (Ba) and ~63 kDa (Bb). The Bb fragment contains the serine protease enzymatic active site. The activated Bb fragment continues activation via the alternative pathway by cleaving, and thus activating, C3 and C5.

Native factor B from human serum. Single-chain glycoprotein present in normal human serum at about 200 µg/ml. During activation of complement via the alternative pathway, Factor B is cleaved and thus activated by Factor D into two fragments of M.W. of 30 kDa (Ba) and 63 kDa (Bb). The Bb fragment contains the serine protease enzymatic active site. The activated Bb fragment continues activation via the alternative pathway by cleaving, and thus activating, C3 and C5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kam, C.M., et al. 1987. J. Biol. Chem. 262, 3444.Gotze, O. and Muller-Eberhard, H.J. 1971. J. Exp. Med. 134, 90s.

Packaging

250 µg in Plastic ampoule

Physical form

In PBS, pH 7.2.

Preparation Note

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)