Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Recombinant, human WEE1 fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.Yoshida, T., et al. 2004. Ann. Oncol.15, 252.Watanabe, N., et al. 1995. EMBO J.14, 1878.Igarashi, M., et al. 1991. Nature353, 80.

Packaging

10 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).

Warning

Toxicity: Standard Handling (A)