Non disponible en dehors du Royaume-Uni et de l'Irlande

Biochem/physiol Actions

ED₅₀ = 0.3-1 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

General description

Recombinant, mouse epiregulin expressed in E. coli with an N-terminal methionine. Epiregulin is shown to inhibit epithelial tumor growth, able to stimulate growth of fibroblasts and other cell types, and has been shown to be an autocrine growth factor in human epidermal keratinocytes.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.Das, S.K., et al. 1997. Dev. Biol.190, 178.Komurasaki, T., et al. 1997. Oncogene15, 2841.Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.Toyoda, H., et al. 1995. FEBS Lett.377, 403.

Packaging

50 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Warning

Toxicity: Standard Handling (A)