Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

This PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸.

Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Jo, M., et al. 2000. J. Biol. Chem.275, 8806.Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.

Packaging

10 tests in Plastic ampoule

Physical form

In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.

Warning

Toxicity: Standard Handling (A)