Non disponible en dehors du Royaume-Uni et de l'Irlande

General description

Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Note: 1 mU = 1 milliunit.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Rao, V., et al. 1995. Structure 3, 449.Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

Packaging

200 miu in Plastic ampoule

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

Warning

Toxicity: Standard Handling (A)